@article { author = {Younis, Sarah and Ali, Wathba}, title = {Identification of polyamine compounds and characterization of polyamine oxidase from sheep's brain tissue}, journal = {Eurasian Chemical Communications}, volume = {3}, number = {9}, pages = {644-655}, year = {2021}, publisher = {Sami Publishing Company (SPC)}, issn = {2717-0535}, eissn = {2676-6280}, doi = {10.22034/ecc.2021.291134.1188}, abstract = {The study included partially purified of polyamine oxidase (PAO) from sheep's brain tissue by dialysis and ion exchange chromatography using DEAE-cellulose techniques. Isoenzymes (I and II) were obtained with specific activities 3.876 and 2.856 units/ mg of protein and purification folds 11 and 8 times respectively compared with crude enzyme.The specific activity of PAO I was better than PAO II, thus we considered it for following studying. The optimal conditions were 40ͦ C and pH=9. Some of properties of partially purified PAO I enzyme were studied and the specificity was found to be superior to the substrate, where it was given the highest activity when using spermidine. The results showed the presence of cupper ion at a concentration of 0.1437 mg / mL in PAO I using an atomic absorption. The presence of the coenzyme conjugate flavin adenine dinucleotide (FAD) was also detected by using UV-Vis spectrum from (325-430) nm.  The inhibitory effect of carbamazepine on the activity of homozygous PAO I was studied, and it was found that there was a decrease in the activity of the enzyme at different inhibitor concentrations. The type of inhibition of the drug was also studied, and it was found non-competitive, and that the value of the inhibition constant Ki was 1.8 mM. The polyamines in the crude extract of Sheep's brain were diagnosed with HPLC technique, and the results of the analysis indicated the presence of spermine, spermidine, cadavarin, diamino propane and hexylamine as well as the presence of other polymines.}, keywords = {Polyamine,Polyamine oxidase,sheep's brain tissue,carbamazepine}, url = {https://www.echemcom.com/article_134802.html}, eprint = {https://www.echemcom.com/article_134802_f7ab375c1b39227f4a4171c54c195533.pdf} }