@article { author = {Salih, Halla and Hamed, Ameera}, title = {Extraction and determination of tannic acid in rosemary, anise, and cinnamon by reversal phase RP-HPLC}, journal = {Eurasian Chemical Communications}, volume = {4}, number = {1}, pages = {94-102}, year = {2022}, publisher = {Sami Publishing Company (SPC)}, issn = {2717-0535}, eissn = {2676-6280}, doi = {10.22034/ecc.2022.318961.1276}, abstract = {A reversed-phase high-performance liquid chromatography method was used for quantitative tannic acid in (rosemary, anise and cinnamon) with isocratic mode. the column which has been used is C18 (250 mm×4.6 mm i.d.;5 µm) at room temperature, with a mobile phase composed of methanol /water which the best ratio for separation tannic acid was (90:10 v: v%) at (pH 6) and the optimum flow rate was 1.5 mL/min, the run time (5 min) at a detection wavelength 277 nm at room temperature. The linearity of calibration curve was (0.1-60 µg mL-1). The value detection of limit and quantification were calculated to be 0.0037 and 0.0122 µg mL-1 respectively. The precision and accuracy were (98.18-100.095%). The recoveries provided by the method were ranged between (-2.57-0.09).}, keywords = {Tannic Acid,polyphenols,UV-Vis,HPLC,Spectrophotometry,Rosemary,Cinnamon,Anise}, url = {https://www.echemcom.com/article_142343.html}, eprint = {https://www.echemcom.com/article_142343_7f5246e749e3c1c07717018f08d86f6a.pdf} }