TY - JOUR ID - 134802 TI - Identification of polyamine compounds and characterization of polyamine oxidase from sheep's brain tissue JO - Eurasian Chemical Communications JA - ECC LA - en SN - 2717-0535 AU - Younis, Sarah Abdulelah AU - Ali, Wathba Idrees AD - Department of Chemistry, College of Education for Pure Science, University of Mosul, Mosul, Iraq Y1 - 2021 PY - 2021 VL - 3 IS - 9 SP - 644 EP - 655 KW - Polyamine KW - Polyamine oxidase KW - sheep's brain tissue KW - carbamazepine DO - 10.22034/ecc.2021.291134.1188 N2 - The study included partially purified of polyamine oxidase (PAO) from sheep's brain tissue by dialysis and ion exchange chromatography using DEAE-cellulose techniques. Isoenzymes (I and II) were obtained with specific activities 3.876 and 2.856 units/ mg of protein and purification folds 11 and 8 times respectively compared with crude enzyme.The specific activity of PAO I was better than PAO II, thus we considered it for following studying. The optimal conditions were 40ͦ C and pH=9. Some of properties of partially purified PAO I enzyme were studied and the specificity was found to be superior to the substrate, where it was given the highest activity when using spermidine. The results showed the presence of cupper ion at a concentration of 0.1437 mg / mL in PAO I using an atomic absorption. The presence of the coenzyme conjugate flavin adenine dinucleotide (FAD) was also detected by using UV-Vis spectrum from (325-430) nm.  The inhibitory effect of carbamazepine on the activity of homozygous PAO I was studied, and it was found that there was a decrease in the activity of the enzyme at different inhibitor concentrations. The type of inhibition of the drug was also studied, and it was found non-competitive, and that the value of the inhibition constant Ki was 1.8 mM. The polyamines in the crude extract of Sheep's brain were diagnosed with HPLC technique, and the results of the analysis indicated the presence of spermine, spermidine, cadavarin, diamino propane and hexylamine as well as the presence of other polymines. UR - https://www.echemcom.com/article_134802.html L1 - https://www.echemcom.com/article_134802_f7ab375c1b39227f4a4171c54c195533.pdf ER -