Web of Science (Emerging Sources Citation Index)

Document Type : Original Research Article


1 Department of R&D, Research and Development Center of Iranian Tissue Product, Tehran, Iran

2 Department of Biotechnology, Malek Ashtar University of Technology (MUT), Tehran, Iran

3 Department of Cellular and Molecular Biology, Faculty of Sciences, Higher Education Institute of Rab-Rashid, Tabriz, Iran

4 Department of Chemometrics, HiTech Institute of Theoretical and Computational Chemistry, India


SDS removal is an important process in proteomics after protein digestion. The research study evaluated an offline SDS removal, as well as, the capability of the offline electrophoretic system for SDS removal. Tyr-Tyr-Tyr, Glu-Val-Phe, and Bradykinin were three peptides to be tested at the presence of the SDS. The electrophoretic system included a 20 µL glass micropipette containing sample which was immersed into acetonitrile at one end and hydrogel at another end. The SDS removal was driven by applying voltage to the system and after removal, the sample inside micropipette can be collected for liquid chromatography, capillary electrophoresis, and mass spectrometry.

Graphical Abstract

Electrophoretic removal of sodium dodecyl sulphate from peptide solution through a hydrogel-micropipette system


Main Subjects

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