Document Type : Original Research Article
- Mohamed Aamer Abubaker 1, 2
- Hamouda Adam Hamouda 3
- Lele Chen 1
- Ling Ding 1
- Sheng Yong Zhu 1
- Ji Zhang 1
- Liang Wan Fu 1
1 College of Life Science, Northwest Normal University, Lanzhou 730070, China
2 Department of Biology, Faculty of Education, University of Khartoum, Khartoum, 11111, Sudan
3 College of Chemistry and Chemical Engineering, Northwest Normal University, Lanzhou, 730070, China
Polysaccharides have multiple significant roles and possess extensive bioactivities in field of medicine, healthcare, food and cosmetic industries, because of their therapeutic effects and relatively minimal toxicity. For the first time, we investigated the chemical structure of Ziziphus spina Christ fruits polysaccharides fraction. The plant is classified as one of the most important Sudanese medical plant, still not adequately researched.
The Z. spina Christi polysaccharides fraction was extracted by using cold water extraction method, deproteinized via Savage’s method, then after participated by using ethanol 80%, the precipitant fractions were dried via lyophilized for 24h. ZSCFPs was investigated by using colorimetric and analytic methods; the colorimetric assays were carried out to determine some functional groups in the polysaccharides fraction such as phenol-sulfuric acid, sulfuric acid - carbazole reaction, ferric chloride reaction. The analytical assays were carried out to confirm the chemical structure of the ZSCFP 80%, which were FTIR spectra, Scanning Electron Microscopy (SEM), Atomic Force Microscopy (AFM), and X-ray diffraction (XRD).