Polysaccharides have multiple significant roles and possess extensive bioactivities in field of medicine, healthcare, food and cosmetic industries, because of their therapeutic effects and relatively minimal toxicity. For the first time, we investigated the chemical structure of Ziziphus spina Christ fruits polysaccharides fraction. The plant is classified as one of the most important Sudanese medical plant, still not adequately researched.
The Z. spina Christi polysaccharides fraction was extracted by using cold water extraction method, deproteinized via Savage’s method, then after participated by using ethanol 80%, the precipitant fractions were dried via lyophilized for 24h. ZSCFPs was investigated by using colorimetric and analytic methods; the colorimetric assays were carried out to determine some functional groups in the polysaccharides fraction such as phenol-sulfuric acid, sulfuric acid - carbazole reaction, ferric chloride reaction. The analytical assays were carried out to confirm the chemical structure of the ZSCFP 80%, which were FTIR spectra, Scanning Electron Microscopy (SEM), Atomic Force Microscopy (AFM), and X-ray diffraction (XRD).