Web of Science (Emerging Sources Citation Index), ISC

Document Type : Original Research Article


Biotechnology and Medicinal Plants Research Center, Ilam University of Medical Sciences, Ilam, Iran


Satureja khuzistanica Jamzad (Lamiaceae) and Thymbra spicata L. (Lamiaceae) are medicinal plants belonging to the genera of Satureja and Thymbra, having many uses in traditional Iranian medicine. S. khuzistanica is used to treat different diseases such as cramps, nausea, and infectious diseases. Medicinal plants, extracts and essential oils are of special importance for pharmacy, health and food industries. S. khuzistanica and T. spicata plants were collected from Dehloran located in south of Ilam province in western Iran. Then, they were dried and powdered. Headspace-solid phase microextraction (HS-SPME) and Gas Chromatography-mass Spectrometry (GC-MS) techniques were used to obtain extracts of mentioned plants and identify their active compounds. Essential oil from T. spicata contained 45 chemical compounds, whose main ones were isothymol (36.24%), gamma-terpinene (17.94%), O-cymene (10.92%), trans-caryophyllene (7.81%), alpha-pinene (7.24%), and camphene (3.61%). Also, essential oil from S. khuzestanica contained 43 chemical compounds which main ones were carvacrol (71.99%), camphene (2.84%), alpha-pinene (1.63%), 1,8-Cineole (1.51%). carvone (1.44%) and beta-pinene (1.42). Results from present study indicated that antioxidative and antimicrobial compounds such as carvacrol were found in both plants (S. khuzestanica and T. spicata) from the Laminaceae family. Therefore, essential oils of these plants can find applications as antioxidant, antimicrobial and antiseptic compounds for medicinal and health purposes.

Graphical Abstract

Extraction and identification of the components of Thymbra spicata L. and Satureja khuzestanica Jamzad Oils native to Ilam province by headspace-solid phase microextraction (HS-SPME) and gas chromatography-mass spectrometry (GC-MS)


Main Subjects

[1] T. Wendimu, Z. Asfaw, E. Kelbessa, J. Ethnopharmacol., 2007, 112, 152–161. [crossref], [Google Scholar], [Publisher]
[2] E. Lulekal, E. Kelbessa, T. Bekele, H. Yineger, J. Ethnobiol. Ethnomedicine, 2008, 4, 10. [crossref], [Google Scholar], [Publisher]
[3] B. Bizuayehu, T. Assefa, J. Med. Plant Res., 2017, 11, 445–454. [crossref], [Google Scholar], [Publisher]
[4] F. Fullybright, Pathogens, 2019, 8(2):73. [crossref], [Google Scholar], [Publisher]
[5] S.L. Chen, H. Yu, H.M. Luo, Q. Wu, C.F. Li, A. Steinmetz, Chin. Med., 2016, 11, 37. [crossref], [Google Scholar], [Publisher]
[6] A.C. Hamilton, Biodivers. Conserv., 2004, 13, 1477–1517. [crossref], [Google Scholar], [Publisher]
[7] I.H.N. Bassolé, H.R. Juliani, Molecules, 2012, 17, 3989–4006. [crossref], [Google Scholar], [Publisher]
[8] A. Ch. Stratakos, A. Koidis, Essential Oils in Food Preservation, Flavor and Safety, 2016, 31-38. [crossref], [Google Scholar], [Publisher]
[9] M. Bahmani, M. Taherikalani, M. Khaksarian, S. Soroush, B. Ashrafi, R. Heydari, Mini Rev. Med. Chem., 2019, 19, 923-932. [crossref], [Google Scholar], [Publisher]
[10] R. Raveau, J. Fontaine, A.L.H. Sahraoui, Foods, 2020, 9, 365. [crossref], [Google Scholar], [Publisher]
[11] A. Orchard, Evid. Based Complement Alternat. Med., 2017, 2017, Article ID 4517971. [crossref], [Google Scholar], [Publisher]
[12] Z. Jamzad, Forests, Rangelands Research Institute of Iran, 2009, 82, 127, 129, 157.
[13] A. Zargari, Medicinal Plants, 4th ed., Tehran University Publications, Tehran, 1990, 42-45. (In Persian)
[14] A. Abdollahi, A.N. Salehnia, S.H.R. Mortazavi, M. Ebrahimi, A. Shafiee, F. Fouladian, K. Keshavarz, S. Sorouri, R. Khorasani, A. Kazemi, Med. Sci. Monit., 2003, 9, 331-335. [Pdf], [Google Scholar], [Publisher]
[15] M. Amanlou, F. Dadkhah, A. Salehnia, H. Farsam, J. Pharmacol. Pharmac. Sci., 2005, 8, 102-106. [Pdf], [Google Scholar], [Publisher]
[16] F. Beyranvand, A. Gharzi, A. Abbaszadeh, RM. Khorramabadi, M. Gholami, AM. Gharravi. Inflamm Regen., 2019, 39, 2. [crossref], [Google Scholar], [Publisher]
[17] A. Brenes, E. Roura, Anim. Feed Sci. Technol., 2010, 158, 1-14. [crossref], [Google Scholar], [Publisher]
[18] M.A. Fellenberg, H. Speisky, Poultry Sci., 2006, 62, 53-70. [crossref], [Google Scholar], [Publisher]
[19] S. Daneshvar-Royandezagh, K.M. Khawar, S. Ozcan,             Biotechnol. Biotechnol. Equip., 2009, 23, 1319–1321. [crossref], [Google Scholar], [Publisher]
[20] A. Miceli, C. Negro, L. Tommasi, Biochem. Syst. Ecol., 2006, 34, 528-535. [crossref], [Google Scholar], [Publisher]
[21] S. Kizil, Ind. Crops Prod., 2010, 32, 593-600. [crossref], [Google Scholar], [Publisher]
[22] T. Kilic, Zeitschrift fur Naturforschung C., 2006, 61, 324-328. [crossref], [Google Scholar], [Publisher]
[23] B. Dousti, J. Plant Res., 2016, 29, 377-384. [Google Scholar], [Publisher]
[24] R. Karousou, DN. Koureas, S. Kokkini, Phytochemistry, 2005, 66, 2668-2673. [crossref], [Google Scholar], [Publisher]
[25] M. Mohammadzarin Abadi, M. Sididi, S.T. Movahedi, 7th Iranian Horticultural Science Congress, 2007, 4-5.
[26] H. Lord, J. Pawliszyn,           J. Chromatogr. A., 2000, 885, 153-193. [crossref], [Google Scholar], [Publisher]
[27] M. Moghaddam, S.N.K. Miran, AG. Pirbalouti, L. Mehdizadeh, Y. Ghaderi, Ind. Crops Prod., 2015, 70, 163–169. [crossref], [Google Scholar], [Publisher]
[28] M. Amiri, D. Esmaeili, A. Sahlehnia, M. Ariana, F. Alam, H. Beiranvand, Int. J. Curr. Microbiol. App. Sci., 2013, 2, 249–254. [Pdf], [Google Scholar], [Publisher]
[29] S. Siavash Saei-Dehkordi, A.A. Fallah, M. Heidari-Nasirabadi, M. Moradi, Int. J. Food Sci. Technol., 2012, 47, 1579–1585. [crossref], [Google Scholar], [Publisher]
[30] M. Yousefzadi, A. Riahi-Madvar, J. Hadian, F. Rezaee, R. Rafiee, M. Biniaz, J Immunotoxicol., 2014, 11, 50–55. [crossref], [Google Scholar], [Publisher]
[31] A. Abbasi, A. Bahador, D. Esmaeili, A. Mahbubi, M. Amiri, M. Amiri, Int. J. Curr. Microbiol. App. Sci., 2014, 3, 614–618. [crossref], [Google Scholar], [Publisher]
[32] D.H. Abou Baker, M. Al-Moghazy, A.A.A. ElSayed, Bioorg Chem., 2020, 95, 103559. [crossref], [Google Scholar], [Publisher]
[33] A.L. Serhat, Y. Yildirim, Ankara Üniv Vet Fak Derg., 2020, 67, 227-233. [crossref], [Google Scholar], [Publisher]
[34] M. Hyldgaard, T. Mygind, R.L. Meyer, Front Microbiol., 2012, 3, 12. [crossref], [Google Scholar], [Publisher]
[35] N. Aligiannis, E. Kalpoutzakis, S. Mitaku, I.B. Chinou, J. Agric  Food Chem., 2001, 49, 4168-4170. [crossref], [Google Scholar], [Publisher]
[36] C.F. Bagamboula, M. Uyttendaele, J. Debevere, Food Microbiol., 2004, 21, 33-42. [crossref], [Google Scholar], [Publisher]
[37] S.G. Deans, K.P. Svoboda, J. Hort. Sci., 1989, 64, 205-210. [crossref], [Google Scholar], [Publisher]
[38] R.S. Farag, Z.Y. Daw, F.M. Hewedi, J. Food Protect., 1989, 52, 665-667. [crossref], [Google Scholar], [Publisher]
[39] S. Kordali, A. Cakir, H. Ozer, R. Cakmakci, M. Kesdek M, E. Mete, Bioresour. Technol., 2008, 99, 8788–8795. [crossref], [Google Scholar], [Publisher]
[40] L. Jovanka, C. Ivana, T. Goran, P. Sava, S. Slavica, C.G. Tamara, K. Ljiljana, Rom. Biotech. Lett., 2011, 16, 6034-6041. [Pdf], [Google Scholar], [Publisher]
[41] N.Z. Mamadalieva, D.K. Akramov, E. Ovidi, A. Tiezzi, L. Nahar, S.S. Azimova, S.D. Sarker, Medicines, 2017, 4, 8. [crossref], [Google Scholar], [Publisher]
[42] K. Kaya, E. Sertkaya, I. Üremiş, S. Soylu, KSU J. Agric. Nat., 2018, 21, 708-714. [crossref], [Google Scholar], [Publisher]
[43] S. Burt, Int. J. Food Microbiol., 2004, 94, 223-253. [crossref], [Google Scholar], [Publisher]
[44] F. Bakkali, S. Averbeck, D. Averbeck, M. Idaomar, Food Chem. Toxicol., 2008, 46, 446-475. [crossref], [Google Scholar], [Publisher]
[45] Z.E. Suntres, J. Coccimiglio, M. Alipour, Crit. Re.v Food Sci. Nutr., 2015, 55, 304-318. [crossref], [Google Scholar], [Publisher]
[46] H. Yang, J. Woo, A.N. Pae, M.Y. Um, N.C. Cho, K.D. Park, M. Yoon, J. Kim, C.J. Lee, S. Cho, Mol. Pharmacol., 2016, 90, 530–539. [crossref], [Google Scholar], [Publisher]
[47] J.A. Klocke, M.V. Darlington, M.F. Balandrin, J. Chem. Ecol., 1987, 13, 2131–2141. [crossref], [Google Scholar], [Publisher]
[48] A.G. Guimarães, J.S.S. Quintans, L.J. Quintans-Júnior, Phytother Res., 2013, 27, 1–15. [crossref], [Google Scholar], [Publisher]
[49] S.S. Dahham, Y.M. Tabana, M.A. Iqbal, M.B.K. Ahamed, M.O. Ezzat, A.S.A. Majid, A.M.S.A. Majid, Molecules, 2015, 20, 11808-11829. [crossref], [Google Scholar], [Publisher]
[50] M. Beránek, D. Nováková, P. Rozsíval, J. Drsata, V. Palicka, Acta Medica., 2006, 49, 35-39. [crossref], [Google Scholar], [Publisher]
[51] G. Bjelakovic, D. Nikolova, L.L. Gluud, R.G. Simonetti, C. Gluud, JAMA., 2007, 297, 842-857. [crossref], [Google Scholar], [Publisher]
[52] J. Peng, GL. Jones, K. Watson, Biol. Med., 2000, 28, 1598-1606. [crossref], [Google Scholar], [Publisher]
[53] Z. Wang, L. Li, M. Song, J. Yan, J. Shi, Y. Yao, J. Ethnopharmacol., 2021, 272, 113957. [crossref], [Google Scholar], [Publisher]  
[54] Z. Wang, L. Li, J. Yan, Y. Yao, IEEE Access, 2020, 8, 82493–82499. [crossref], [Google Scholar], [Publisher]  
[55] Y. Yao, Z. Wang, L. Li, K. Lu, R. Liu, Z. Liu, J. Yan, Comput. Math. Methods Med., 2019, 2019, 8617503. [crossref], [Google Scholar], [Publisher]  
[56] M. Momtaz, M. Abdollahi, Pharmacologyonline, 2008, 2, 34-54. [Google Scholar], [Publisher]  
[57] V. Tolan, S. Kızıl, S. Özdemir, O. Demirci, Fresenius Environ. Bull.., 2009, 18, 533-537. [Pdf], [Google Scholar], [Publisher]