%0 Journal Article %T Identifying profiles of SSR and SNP markers in cultivars of tetraploid wheat: physical and chemical analysis %J Eurasian Chemical Communications %I Sami Publishing Company (SPC) %Z 2717-0535 %A Yousefi Javan, Iman %A Augusto Pagnotta, Mario %A D΄Ovidio, Renato %D 2020 %\ 04/01/2020 %V 2 %N 4 %P 516-535 %! Identifying profiles of SSR and SNP markers in cultivars of tetraploid wheat: physical and chemical analysis %K genome %K SSR %K Wheat %K Tetraploid %R 10.33945/SAMI/ECC.2020.4.9 %X In this work, we studied the distribution of molecular markers in the chromosomes of tetraploid wheat. This distribution was drawn through the 192 line came from based on a cross between two durum wheat genotypes. The first parental line was a Triticum turgidum ssp. durum (Desf.). The second parental line was a durum wheat genotype derived from a cross between the Triticum turgidum ssp. Durum, (Omrabi 5). One hundred ninety-two F8 recombinant inbred lines (RILs) derived from the above mentioned cross by single-seed descent. A total of 254 markers were analyzed, including 216 microsatellites and 38 SNPs markers. Linkage analysis defined 14 linkage groups. Most markers (57.2%) were found to be located to the A genome, with an average of 12 markers per chromosome. The remaining (42.7%) were located to the B genome. To construct a stabilized (skeleton) map, markers interfering with map stability were removed. Efficient user-friendly methods for mapping plant genomes were highly desirable for the studies marker-assisted selection. SSR (microsatellite) markers are user-friendly and efficient in detecting polymorphism, but they detect few loci. The skeleton map consisted of 100 markers with a total length of 3170.29 cM and an average distance of 31.7 cM between adjacent markers. Majority of the markers showed a statistical significantly Mendelian segregation with 1:1 ratio (α=0.01). The highest percentage of markers was similar with the first parental. This SSR and SNP markers revealed a high proportion of clustering, which may be indicative of gene-rich regions. Some of the SSR, SNP markers were distributed for the first time on the current work. This project provided a useful groundwork for further genetic map, genetic analysis of important quantitative traits, positional cloning, and marker-assisted selection, as well as for genome comparative genomics and genome organization studies in wheat and other cereals. %U https://www.echemcom.com/article_99343_793ae41be3ada165864d6a6328517ed6.pdf