Document Type : Original Research Article

Authors

• Simin Sharifi ¹
• Farzaneh Lotfipour ^{2, 3}
• Mohammad Ali Ghavimi ⁴
• Solmaz Maleki Dizaj ¹
• Shahriar Shahi ¹
• Javad Yazdani ⁴
• Masumeh Mokhtarpour ⁵
• Rovshan Khalilov ^{6, 7, 8}

¹ Dental and Periodontal Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

² Food & Drug Safety Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

³ Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran

⁴ Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Tabriz University of Medical Sciences, Tabriz, Iran

⁵ Department of Physical Chemistry, University of Tabriz, Tabriz, Iran

⁶ Department of Biophysics and Molecular Biology, Baku State University, Baku, Azerbaijan

⁷ Institute of Radiation Problems, National Academy of Sciences of Azerbaijan, Baku, Azerbaijan

⁸ International Research and Education Center of Nanobiotechnology and Functional Nanosystems, Drohobych Ukraine & Baku, Azerbaijan

Abstract

In this study, HAP-gelatin and CC-gelatin nanocomposite scaffolds, as bioactive inorganic materials, were synthesized successfully through a chemical precipitation procedure. Next, characterization of the prepared nanocomposite scaffolds was completed using scanning electron microscopy (SEM), dynamic light scattering (DLS), zeta-sizer (for zeta potential measurement), X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR). Then, we soaked the generated nanocomposite scaffolds in the simulated body fluid (SBF) for several times to investigate and compare the bioactivity of these nanocomposites and determine the percent of weight loss. The rate of calcium ions dissolution in SBF media was determined utilizing atomic absorption spectroscopy (AAS). The findings of characterization showed that the preparation of nanocomposites was successful with monodispersed nanosized particles, uniform agglomerated morphology, crystalline form, and negative surface charge. According to the results of the bioactivity test, both nanocomposite scaffolds were of high bioactivity, corroborated well with the patterns of calcium release. Calcium ions released from the HAP-gelatin nanocomposite were higher than that of the CC-gelatin. However, the bioactivity of CC was comparable with well-known bioactive HAP material. Therefore, it could be a promising alternative for use compared with HAP, the preparation of which is more complicated and expensive.

Graphical Abstract

Keywords

• Hydroxyapatite
• calcium carbonate
• gelatin
• nanocomposite scaffold
• bioactivity
• SBF

Main Subjects

• Application of nanomaterial