Scopus (CiteScore 2022 =3.0, Q3) , ISC

Document Type : Original Research Article


Department of Chemistry, College of Science for Women, University of Baghdad, Baghdad, Iraq



From Streptomyces species isolated from agricultural soil, an extracellular L-glutamate oxidase was recovered. The Streptomyces bacterial isolates were grown in a selective medium that had L-glutamate as a substrate. The filtrate, which is representative of the crude enzyme, was subsequently obtained by extracting the extracellular enzyme using a cooling centrifugation procedure. L-glutamate oxidase purification operations were then performed, starting with fractionation with 40% ammonium salts and continuing with ion-exchange chromatography and gel filtration with a final 8.25 units/mg and 61.8% yield. Both positive and negative bacteria were sensitive to the pure L-glutamate oxidase's effects. Purified L-glutamate oxidase outperformed the other examined bacterial isolates in terms of activity, with MIC values of 32 µg/ml for Klebsiella pneumonia and 64 µg/ml for Staphylococcus aureus and Escherichia coli. Thus, pure L-glutamate oxidase may be viewed as a promising possibility in the rational design of new antibiotics.

Graphical Abstract

An antibacterial activity of the produced and purified L-glutamate oxidase from Streptomyces sp


Main Subjects

[1] M. Barakate, Y. Ouhdouch, K.H. Oufdou, C. Beaulieu, Characterization of rhizospheric soil Streptomycetes from Moroccan habitats and their antimicrobial activities, World J. Microbiol. Biotechnol., 2018, 18, 49–54. [crossref], [Google Scholar], [Publisher]
[2] H.S. Chaudhary, J. Yadav, A.R. Shrivastava, S. Singh, A.K. Singh, N. Gopalan, Antibacterial activity of actinomycetes isolated from different soil samples of Sheopur (A city in central India). JAPTR, 2017, 4, 118-23. [crossref], [Google Scholar], [Publisher]
[3] G.A. Quinn, A.M. Banat, M. Abdelhameed, I.M. Banat, Streptomyces from traditional medicine: Sources of innovation in antibiotic discovery, JMM, 2020, 69, 1040.  [crossref], [Google Scholar], [Publisher]
[4] K.F. Chater, Recent advances in understanding Streptomyces, F1000Research, 2016, 5, 16-21. [crossref], [Google Scholar], [Publisher]
[5] S. Wachiratianchai, A. Bhumiratana, S. Udomsopagit, Isolation, purification, and characterization of L-glutamate oxidase from Streptomyces sp. 18G, Electron. J. Biotechnol., 2014, 7, 09-10. [Google Scholar], [Publisher]
[6] V.M. Almeida, S.R. Marana, The optimum temperature may be a misleading parameter in enzyme characterization and application, PloS one, 2019, 14, e0212977. [crossref], [Google Scholar], [Publisher]
[7] H. Maleki, O. Mashinchianm, Characterization of streptomyces isolates with UV, FTIR spectroscopy, and HPLC analyses, Bioimpacts, 2020, 1, 47-52. [crossref], [Google Scholar], [Publisher]
[8] R. Chau, J.A. Kalaitzis, S.A. Wood, B.A. Neilan, Diversity and biosynthetic potential of culturable microbes associated with toxic marine animals, Marine drugs, 2018, 11, 2695-2712. [crossref], [Google Scholar], [Publisher]
[9] A.E. Murray , J. Freudenstein, S. Gribaldo , R. Hatzenpichler , P. Hugenholtz , P. Kämpfer,  et al., Roadmap for naming uncultivated Archaea and Bacteria, Nat. Microbiol., 2020, 5, 987–994. [crossref], [Google Scholar], [Publisher]
[10] L. Qingshan, W. Lijun, L. Yourong, Color development with rational screening method for improved L-glutamate oxidase-producing strains, E MT, 2009, 18, 7-9. [crossref], [Google Scholar], [Publisher]
[11] T. Oikawa, M. Watanabe, H. Makiura, H. Kusakabe, K. Yamade, K. Soda, Production of D-glutamate from L-glutamate with glutamate racemase and L-glutamate oxidase, BBB, 2009, 63, 2168-2173. [crossref], [Google Scholar], [Publisher]
[12] M. Bradford, A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding, Anal. Biochem., 1976, 72, 248-254. [crossref], [Google Scholar], [Publisher]
[13] A.P. Chowdhury, R.R. Singh, B. Bharadwaj, M.P. Bhuyana, S. Jyoti, Qualitative assessment on isolated tartar forming bacteria from dental caries by pattern of screening, assembling and antibiotic sensitivity, JRMDS, 2019, 7, 140-157. [crossref], [Google Scholar], [Publisher]
[14] R.F. Seipke, M. Kaltenpoth, M.I. Hutchings, Streptomyces as symbionts: An emerging and widespread theme?, FEMS Microbiol. Rev., 2020, 36, 862–876. [crossref], [Google Scholar], [Publisher]
[15] J.T. Newitt, S.M.M. Prudence, M.I. Hutchings, S.F. Worsley, Biocontrol of cereal crop diseases using streptomycetes, Pathogens, 2019, 8, 78-82. [crossref], [Google Scholar], [Publisher]
[16] F.M. Rashad, H.M. Fathy, A.S. El-Zayat, A.M. Elghonaimy, Isolation and characterization of multifunctional Streptomyces species with antimicrobial, nematicidal, and phytohormone activities from marine environments in Egypt, Microbiol. Res., 2016, 175, 34–47. [crossref], [Google Scholar], [Publisher]
[17] Q.S. Li, J.J. Zhong, Short communication: Production of L-glutamate oxidase by Streptomyces sp. N1 in submerged fermentation, WJMB, 2011, 12, 651-658. [crossref], [Google Scholar], [Publisher]
[18] Q.S. Li, Q.H. Fang, J.Y. Zhu, J.J. Zhong, Hyperproduction of L-glutamate oxidase in submerged fermentation of Streptomyces sp. N1 with culture pH control and calcium addition, ABAB, 2014, 80, 97-106. [crossref], [Google Scholar], [Publisher]
[19] H. Abdul Hameed, H. Ali, Extraction and purification of extracellular L-glutamate oxidase from Streptomyces, Archives of Razi Institute, 2021, 76, 769-779. [crossref], [Google Scholar], [Publisher]
[20] H. Kusakabe, Y. Midorikawa, A. Kuninaka, H. Yoshino, Occurrence of a new enzyme, l-glutamate oxidase in a wheat bran culture extract of Streptomyces sp. X-119-6, Agric. Biol. Chem, 2017, 47, 179-82. [crossref], [Google Scholar], [Publisher]
[21] S.F. Jasim, Y.F. Mustafa, A review of classical and advanced methodologies for benzocoumarin synthesis, J. Med. Chem. Sci, 2022, 5, 676-694. [crossref], [Google Scholar], [Publisher]
[22] D.D. Belova, E.N. Kharchenko, O.S. Chaplygina, Identification of residual traces of antibiotics in food, J. Med. Chem. Sci, 2022, 5, 385-392. [crossref], [Google Scholar], [Publisher]
[23] Y.F. Mustafa, Classical approaches and their creative advances in the synthesis of coumarins: A brief review, J. Med. Chem. Sci, 2021, 4, 612-625. [crossref], [Google Scholar], [Publisher]