Major flavonoids identification by HPLC-MS/MS and evaluation of antiradical potential of mung bean (Vigna radiata) seed extracts in Burkina Faso

Ouedraogo, Mahamadi; Bazie, Benjamin; Kabore, Dominique Saga; Ouedraogo, Salfo; Bazie, Bazoin Sylvain Raoul; Kabre, Elie; Zida, Wend-Pagnagde Félicien Marie Serge; Batieno, Benoit Joseph T.; Koala, Moumouni; Pale, Eloi

doi:10.22034/ecc.2023.398139.1650

Document Type : Original Research Article

Authors

Mahamadi Ouedraogo ¹^{, 2}
Benjamin Bazie ¹^{, 3}
Dominique Saga Kabore ¹
Salfo Ouedraogo ⁴^{, 5}
Bazoin Sylvain Raoul Bazie ⁶^{, 7}
Elie Kabre ⁷
Wend-Pagnagde Félicien Marie Serge Zida ⁸
Benoit Joseph T. Batieno ⁸
Moumouni Koala ¹^{, 4}
Eloi Pale ¹

¹ Laboratoire de Chimie Organique et de Physique Appliquées, Département de Chimie, UFR-SEA, Université Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina Faso

² Université Norbert ZONGO, avce Maurice Yameogo, Koudougou BP 376, Burkina Faso

³ École Polytechnique de Ouagadougou, Ouagadougou, Burkina Faso

⁴ Laboratoire de Recherche-Développement de Phytomédicaments et Médicaments, Département Médecine, Pharmacopée Traditionnelles et Pharmacie, Institut de Recherche en Science de la Santé (IRSS/CNRST), 03 BP 7047 Ouagadougou, Burkina Faso

⁵ Centre de Formation, de Recherche et d’Expertise en Sciences du Médicament (CEA-CFOREM), Département sciences et santé, Université Joseph KI-ZERBO, 03 BP 7021 Ouagadougou, Burkina Faso

⁶ Centre Universitaire de Manga, Département de Biochimie, Manga, Burkina Faso

⁷ Agence Nationale pour la Sécurité Sanitaire de l’Environnement, de l’Alimentation, du Travail et des produits de santé (ANSSEAT), Département de Biochimie, Ouagadougou, 09 BP Ouagadougou 09, Burkina Faso

⁸ Laboratoire de Génétique et de Biotechnologies Végétales, Département de Productions Végétales, Institut de l’Environnement et des Recherches Agricoles (INERA) 04 BP 8545 Ouagadougou, Burkina Faso

10.22034/ecc.2023.398139.1650

Abstract

Mung bean is one of the most essential legumes in Burkina Faso due to its short maturation cycle (45 to 55 days) and its nutritional content. This work aims to contribute to a better knowledge of the bioactive compounds of the seeds of Beng Tigré, a mung bean cultivar originating from India and acclimatized in Burkina Faso. To do this, the contents of total phenolic compounds, flavonoids, and carotenoids were determined by colorimetric methods. The mineral elements were assayed by atomic absorption spectrometry after the seeds mineralization. The antiradical potential was evaluated by monitoring the disappearance of the stable radical DPPH• by UV-Visible absorption spectrophotometry at 517 nm and compared to standards. Major flavonoids were identified by high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). Total phenolic compounds, flavonoids, and carotenoids were 4. 356±0.162 mg GAE/g, 3. 151±0.262 mg QE/g, and 0.302±0.012 mg BCE/g, respectively. Potassium, magnesium, and calcium were, respectively, the most abundant mineral elements in the seeds: K > Mg > Ca > Fe > Zn > Na > Mn > Cu. An antiradical activity of the Beng Tigré extract was observed with an IC₅₀ of 209. 97±13. 55 µg of AO/g DPPH•. This activity is less effective than Gallic acid, ascorbic acid, and trolox. Finally, the HPLC-MS/MS analysis identified four compounds: naringenin 8-C-glucoside, luteolin 8-C-glucoside, apigenin 6-C-glucoside, and luteolin 7-O -rutinoside. Due to their high biological potential, these so-called bioactive compounds are the subject of growing research interest.

Graphical Abstract

Keywords

Main Subjects

Organic Chemistry

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Eurasian Chemical Communications

Major flavonoids identification by HPLC-MS/MS and evaluation of antiradical potential of mung bean (Vigna radiata) seed extracts in Burkina Faso

References

References

Volume 5, Issue 10
October 2023
Pages 935-952

Major flavonoids identification by HPLC-MS/MS and evaluation of antiradical potential of mung bean (Vigna radiata) seed extracts in Burkina Faso

References

References

Volume 5, Issue 10October 2023Pages 935-952

Volume 5, Issue 10
October 2023
Pages 935-952