Scopus (CiteScore 2022 =3.0, Q3) , ISC

Document Type : Original Research Article


1 Department of Pharmacy, Faculty of Health Science, University of Muhammadiyah Malang, Malang, Indonesia

2 Department of Pharmacy, Faculty of Medicine and Health Science, Maulana Malik Ibrahim State Islamic University, Malang, Indonesia



Pain and inflammation in the joints from cartilage breakdown characterize osteoarthritis (OA), a degenerative disease linked to low estrogen levels. Because phytoestrogens can perform the same role as estrogen, they may be useful in relieving OA-related pain and inflammation. It is well-documented that some plants, like Chrysophyllum cainito L., contain phytoestrogens. To identify the efficacy of a 70% ethanol extract (CLE) and tablet (CLT) formulated from C. cainito leaves in reducing pain perception in male Wistar rats. Acute toxicity tests were initially carried out on the CLE in rats. The analysis was carried out for 14 days, and then a probit analysis was performed to determine the LD50 value. The antinociceptive activity of CLE and CLT was then tested on rats using the writhing test at doses of 4.05, 8.1, and 16.2 mg/200 g BW rat/day for the CLE, and 24.3, 48.6, and 97.2 mg/200 g BW rat/day for the CLT. After 30 minutes of administration of each sample, the rats were induced intraperitoneally with 1% acetic acid and then observed for their stretch for 30 minutes. The LD50 for the CLE was 70.028 g/kg, indicating that it was practically non-toxic. The rats also showed no signs of toxicity qualitatively. Both the CLE and CLT demonstrated antinociceptive activity, with the optimal dose for CLE being 4.05 mg/200 g BW, and the optimal dose for CLT being 48.6 mg/200 g BW, resulting in percentages of writhing inhibition of 80.61 ± 7.3 and 80.62 ± 7.3, respectively. These results indicate that CLE and CLT are safe and have an antinociceptive effect, so they have the potential to be developed as alternative anti-OA drugs.

Graphical Abstract

The antinociceptive effect of Chrysophyllum cainito L. leaves extract and tablet in wistar rats


Main Subjects

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