Scopus (CiteScore 2022 =3.0, Q3) , ISC

Document Type : Original Research Article


1 Department of Conservative Dentistry, Faculty of Dentistry Airlangga University, Surabaya, Indonesia

2 onservative Dentistry Specialist Program, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia

3 Undergraduate Program, Faculty of Dentistry Airlangga University, Surabaya, Indonesia



In dental pulp tissue, lipopolysaccharide invasion may be the cause of nerve cell death, which lowers the total amount of nerve cells in the pulp. The disease causes the pulp of teeth to deteriorate, which may have an impact on the risk of endodontic flare-ups. One sort of cell death used to lower the quantity of undesirable cells that might compromise pulp quality is called necroptosis. The goal of this study is to understand how Porphyromonas gingivalis lipopolysaccharide injection causes the necroptosis of dental pulp nerve cells. For experimental section, 32 Sprague Dawley rats in total were divided into 2 groups. Lower incisor access was induced in the treatment group using Porphyromonas gingivalis lipopolysaccharide, whereas in the control group, lower incisor access was performed and filled with GIC. A day later, both groups were closed. Next, preparation slides were made, and using immunohistochemical labeling, the number of nerve cells and Mixed Lineage Kinase Domain-Like Protein (MLKL) was measured. The findings demonstrate a substantial difference between the lipopolysaccharide treatment group and the control group, as well as an increase in MLKL expression and a decrease in nerve cell count following lipopolysaccharide injection. Finally, this study shows that the injection of Porphyromonas gingivalis lipopolysaccharide results in necroptosis in dental pulp tissue nerve cells.

Graphical Abstract

Necroptosis after lipopolysaccharide administration based on expression of mixed lineage kinase domain-like protein and number of nerve cells in dental pulp tissue


Main Subjects

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