An antibacterial activity of the produced and purified L-glutamate oxidase from Streptomyces sp

Nasser Fadhel, Suha; Slman Dawood, Ashgn

Document Type : Original Research Article

Authors

Department of Chemistry, College of Science for Women, University of Baghdad, Baghdad, Iraq

Abstract

From Streptomyces species isolated from agricultural soil, an extracellular L-glutamate oxidase was recovered. The Streptomyces bacterial isolates were grown in a selective medium that had L-glutamate as a substrate. The filtrate, which is representative of the crude enzyme, was subsequently obtained by extracting the extracellular enzyme using a cooling centrifugation procedure. L-glutamate oxidase purification operations were then performed, starting with fractionation with 40% ammonium salts and continuing with ion-exchange chromatography and gel filtration with a final 8.25 units/mg and 61.8% yield. Both positive and negative bacteria were sensitive to the pure L-glutamate oxidase's effects. Purified L-glutamate oxidase outperformed the other examined bacterial isolates in terms of activity, with MIC values of 32 µg/ml for Klebsiella pneumonia and 64 µg/ml for Staphylococcus aureus and Escherichia coli. Thus, pure L-glutamate oxidase may be viewed as a promising possibility in the rational design of new antibiotics.

Graphical Abstract

Keywords

Main Subjects

Biochemistry

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Eurasian Chemical Communications

An antibacterial activity of the produced and purified L-glutamate oxidase from Streptomyces sp

References

References

Volume 5, Issue 10
October 2023
Pages 907-914

An antibacterial activity of the produced and purified L-glutamate oxidase from Streptomyces sp

References

References

Volume 5, Issue 10October 2023Pages 907-914

Volume 5, Issue 10
October 2023
Pages 907-914